Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV006354 GenBank Accession Z95629
Subfamily Parvovirinae Genus Erythroparvovirus
Species Erythroparvovirus primate1 Sequence Length 334
Definition Erythrovirus B19 P6 promoter region (Patient K). Submission Date 1997-05-20
Strain Name Patient K Sampling Date
Sampling Country None Location None
Submitted By Gareus,R. Submitting Institution Gareus R., Institut fuer Med. Mikrobiologie u. Hygiene Uni Regensburg, Franz-Josef-Strauss Allee 11, Regensburg, GERMANY, 93053
Original Host human Sample Type sera
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Patients' sera Provided source material for amplification of B19 virus promoter regions. 9420265 View Related Publication
2 Nested PCR primers (forward and backward primers) Used to amplify B19 virus promoter regions from sera with low virus load.
3 373A Sequencer (Applied Biosystems) Used for sequencing the PCR products.
4 Fetal calf serum Used as a supplement in the culture media for cell lines.
5 Dulbecco’s modified Eagle’s medium Growth medium for HeLa cells.
6 Sodium pyruvate Added to HeLa cell growth medium.
7 Glutamine Added to HeLa cell growth medium.
8 Penicillin Used as an antibiotic in cell culture media.
9 Streptomycin Used as an antibiotic in cell culture media.
10 Hygromycin B Used to select for HeLa cells containing the episomal vector pGRE5-2/EBV-NS or pGRE.
11 Dulbecco’s RPMI 1640 Growth medium for BJAB, CEM, and K562 cells.
12 Kanamycin Used as an antibiotic in cell culture media.
13 Expedite solid-phase synthesis system (Millipore) Synthesized primers for PCR amplification.
14 NheI and HindIII restriction sites Facilitated cloning of PCR-amplified promoter constructs into the luciferase vector pGL-3 Basic.
15 pGL-3 Basic (Promega Corporation) Vector used for cloning B19 virus promoter fragments upstream of the luciferase gene.
16 Renilla luciferase vector pRL-CMV (Promega) Used as an internal reference for luciferase activity assays.
17 Dexamethasone (Sigma-Aldrich) Induced expression of NS1 gene under control of glucocorticoid response element in HeLa/NS-21 cells.
18 Passive lysis buffer (Promega) Used for cell lysis prior to luciferase activity determination.
19 Luciferase assay system (Promega) Enabled measurement of luciferase activity using a luminometer.
20 Luminometer (Lumat LB9501; EG&G Berthold) Used for reading luciferase activity from lysate samples.
21 Electroporator (EasyJect Plus, Eurogentec) Used for introducing plasmids into BJAB, CEM, and K562 cells by electroporation.