Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV006235 | GenBank Accession | AY530588 |
| Subfamily | Parvovirinae | Genus | Dependoparvovirus |
| Species | Dependoparvovirus primate1 | Sequence Length | 2208 |
| Definition | Adeno-associated virus isolate hu.22 capsid protein VP1 (cap) gene, complete cds. | Submission Date | 2004-01-20 |
| Strain Name | hu.22 | Sampling Date | |
| Sampling Country | United States of America | Location | United States of America |
| Submitted By | Gao,G., Vandenberghe,L.H., Alvira,M.R., Lu,Y., Calcedo,R., Zhou,X. and Wilson,J.M. | Submitting Institution | Gene Therapy Program, Division of Medical Genetics, Department of Medicine, University of Pennsylvania, 3601 Spruce Street, Wistar Institute, Philadelphia, PA 19104, USA |
| Original Host | Homo sapiens | Sample Type | tissue samples |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | Human tissues | Collected from surgical procedures, postmortem examinations, or organ donors for AAV detection. | 15163731 | View Related Publication |
| 2 | Total cellular DNAs | Extracted from human and nonhuman primate tissues for PCR analysis of AAV sequences. | ||
| 3 | Primer sets for cap gene (not specified) | Used for signature or full-length cap PCRs to determine the molecular prevalence and tissue distribution of AAVs. | ||
| 4 | Capsid clones (hu.1 to hu.67, among others) | Isolated and sequenced from human tissues to characterize AAV capsid sequences. | ||
| 5 | Polyclonal antisera against AAV serotypes | Generated for cross-neutralizing reactivities testing of AAV vectors. | ||
| 6 | Enhanced green fluorescent protein (EGFP) vectors | Used to examine transduction efficiencies in vitro in 84-31 cells. | ||
| 7 | Human α1-antitrypsin (A1AT) vectors | Used as a sensitive and quantitative reporter gene in vivo, expressed under the control of a cytomegalovirus-enhanced chicken β-actin promoter. | ||
| 8 | 4 to 6-week-old NCR nude mice | Used as subjects for in vivo studies involving liver-, lung-, and muscle-directed gene transfer. | ||
| 9 | Enzyme-linked immunosorbent assay (ELISA) kits (for A1AT) | Used to determine serum A1AT concentrations following gene transfer in vivo. |