Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV006147 | GenBank Accession | KM073055 |
| Subfamily | Parvovirinae | Genus | Erythroparvovirus |
| Species | Erythroparvovirus primate1 | Sequence Length | 335 |
| Definition | Human parvovirus B19 isolate BRRJ_HSCT_D8 non-structural protein 1 (NS1) gene, partial cds. | Submission Date | 2014-06-24 |
| Strain Name | BRRJ_HSCT_D8 | Sampling Date | 2012 |
| Sampling Country | Brazil | Location | Brazil |
| Submitted By | Gama,B.E. and Hassan,R. | Submitting Institution | Bone Marrow Transplantation Center, National Institute of Cancer (INCA), Praca da Cruz Vermelha 23, Rio de Janeiro, RJ 20230130, Brazil |
| Original Host | Homo sapiens | Sample Type | DNA samples |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | DNAzol reagent | Isolation of genomic DNA from peripheral blood (PB) and bone marrow (BM) cells of healthy donors. | 29184906 | View Related Publication |
| 2 | Ficoll-Paque Plus (GE Healthcare and Life Sciences) | Processing of cells from PB or BM from pretransplantation and posttransplantation samples. | ||
| 3 | Platinum Taq DNA polymerase and reagents (Invitrogen) | Conducting polymerase chain reaction (PCR) assays. | ||
| 4 | Wizard SV Gel and PCR Clean-Up System (Promega) | Purification of PCR products from the second round of amplification. | ||
| 5 | Big Dye Terminator Cycle Sequencing Ready Reaction version 3.1 (Life Technologies) | DNA sequencing from forward and reverse strands. | ||
| 6 | pCR 2.1 TOPO TA vector (Invitrogen, Life Technologies) | Molecular cloning of B19V+ amplified fragments. | ||
| 7 | QIAprep Spin Miniprep Kit (Qiagen) | Isolation and purification of plasmidial DNA. |