Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV006005 | GenBank Accession | AB514453 |
| Subfamily | Parvovirinae | Genus | Bocaparvovirus |
| Species | Bocaparvovirus primate1 | Sequence Length | 354 |
| Definition | Human bocavirus NP1 gene for NP1 protein, partial cds, strain: PHL06-165. | Submission Date | 2009-07-30 |
| Strain Name | PHL06-165 | Sampling Date | 2006 |
| Sampling Country | Philippines | Location | Alabang,Muntinlupa, Philippines |
| Submitted By | Furuse,Y., Suzuki,A., Kishi,M., Galang,H.O., Lupisan,S.P., Olveda,R.M. and Oshitani,H. | Submitting Institution | Contact:Akira Suzuki Tohoku University Graduate School of Medicine, Department of Virology; 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575, Japan |
| Original Host | Homo sapiens | Sample Type | nasopharyngeal aspirates |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | Nasopharyngeal aspirates | Collected from patients exhibiting influenza-like illnesses at sentinel health care facilities. | 20419824 | View Related Publication |
| 2 | MDCK and HEp-2 cells | Used for inoculation and viral culture to detect influenza A and B viruses, adenovirus, enterovirus, herpes simplex virus type 1 (HSV-1), parainfluenza viruses 1 and 3, rhinovirus, and RSV. | ||
| 3 | PureLink Viral RNA/DNA MiniKit (Invitrogen, Carlsbad, CA) | Used for RNA extraction from stored samples. | ||
| 4 | Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase | Used to reverse transcribe viral RNA to complementary DNA. | ||
| 5 | Random hexamer primers | Used for reverse transcription of viral RNA. | ||
| 6 | Agarose gels | Used to separate all amplified products from PCR assays. | ||
| 7 | BigDye Terminator version 1.1 (Applied Biosystems, Foster City, CA) | Used for sequencing PCR-amplified fragments. |