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1
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Primers PV4ORF1F (5′-AAGACTACATACCTACCTGTG-3′) and PV4ORF1R (5′-GTGCCTTTCATATTCAGTTCC-3′)
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Designed to amplify a 220-bp region of the ORF1 of PARV4.
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16494735
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View Related Publication
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2
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1× PCR buffer II (PE Applied Biosystems, Warrington, UK)
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Buffer used in PCR reactions.
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3
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200 μmol/L each deoxynucleoside triphosphate
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Building blocks for DNA synthesis in PCR.
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4
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2 mmol/L MgCl2
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Cofactor required for the DNA polymerase enzyme activity during PCR.
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5
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10 pmol each primer
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Primers for initiating DNA synthesis in PCR.
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6
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2.5 U AmpliTaq Gold DNA polymerase (PE Applied Biosystems)
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DNA polymerase enzyme used in PCR.
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7
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Agarose gel electrophoresis
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Method to analyze PCR amplicons and compare them to known size markers.
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8
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pT7 Blue vector (Novagen, Darmstadt, Germany)
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Vector used for cloning the PARV4 control sequences.
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9
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LightCycler FastStart DNA MasterPLUS SYBR green I kit (Roche Applied Science, Mannheim, Germany)
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Kit used to perform real-time PCR for determining levels of PARV4 DNA.
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