Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV005882 GenBank Accession DQ873386
Subfamily Parvovirinae Genus Tetraparvovirus
Species Tetraparvovirus primate1 Sequence Length 4949
Definition Human parvovirus 4 isolate BR10749-4 ORF1 and ORF2 genes, complete cds. Submission Date 2006-07-31
Strain Name BR10749-4 Sampling Date from 2004 to 2005
Sampling Country United States of America Location United States of America
Submitted By Fryer,J.F., Delwart,E., Lukashov,V.V. and Baylis,S.A. Submitting Institution Division of Virology, National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar, Hertfordshire EN6 3QG, United Kingdom
Original Host Homo sapiens Sample Type plasma samples
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Phusion HF Buffer (Finnzymes OY) Used as a buffer in the reaction mixture 17622618 View Related Publication
2 dNTP Nucleotides used in the PCR reaction
3 Primers PARV4/5seq1, PVORF2R, PVORF2F, PARV4/5seq21 Primers used for amplifying cDNA fragments spanning ORF1 and ORF2 coding regions
4 Phusion Hot Start DNA Polymerase (Finnzymes OY) Enzyme used in the PCR reaction to amplify DNA
5 pT7 Blue vector (Novagen) Vector used for cloning PCR products
6 Hybond XL membrane (GE Healthcare) Membrane used for immobilizing denatured DNA
7 Rapid-hyb buffer (GE Healthcare) Buffer used for pre-hybridization of membranes
8 [γ-32P]ATP Radioactive label used for 5'-end labeling of oligonucleotides
9 T4 polynucleotide kinase (TaKaRa) Enzyme used for labeling oligonucleotides with [γ-32P]ATP
10 SSC (saline-sodium citrate) Used in the washing solution for membranes after hybridization
11 SDS (sodium dodecyl sulfate) Component of the washing solution for hybridization membranes