Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV005483 GenBank Accession AF272985
Subfamily Parvovirinae Genus Erythroparvovirus
Species Erythroparvovirus primate1 Sequence Length 622
Definition B19 virus non-structural protein NS1 gene, partial cds. Submission Date 2000-05-30
Strain Name None Sampling Date
Sampling Country None Location None
Submitted By Brunstein,J.D., Soderlund-Venermo,M. and Hedman,K.P. Submitting Institution Virology, Haartman Institute, University of Helsinki, Haartmaninkatu 3, Helsinki 00290, Finland
Original Host human Sample Type Serum samples
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 RPMI 1640 Culture medium for the human megakaryoblastoid cell line MB-02. 10964772 View Related Publication
2 Glutamine Supplement added to the culture medium.
3 Penicillin Antibiotic added to the culture medium.
4 Streptomycin Antibiotic added to the culture medium.
5 Gentamicin (Sigma-Aldrich Co., 20 mg/ml) Antibiotic added to the culture medium.
6 GM-CSF (Boehringer Mannheim Biochemica, 200 U/ml) Growth factor added to the culture medium.
7 Fetal calf serum (GIBCO BRL, 10%) Supplement added to the culture medium.
8 Erythropoietin (Epo, Boehringer Mannheim, 4 U/ml) Induces erythroid differentiation of MB-02 cells.
9 Stem cell factor (SCF, Boehringer Mannheim, 25 ng/ml) Induces erythroid differentiation of MB-02 cells.
10 Trizol reagent (GIBCO BRL) Isolation of total RNA from cell culture.
11 Titan one-tube system (Boehringer Mannheim) RT-PCR reagent for reverse transcription and amplification.
12 Amplitaq Gold polymerase (Perkin-Elmer) Reagent for seminested second-round PCR amplifications.
13 DNAse-free RNase A (Sigma-Aldrich) Digestion of RNA samples to confirm RNA template origin.
14 Proteinase K Digests proteins to help isolate viral DNA.
15 Prep-A-Gene kit (BioRad Laboratories) Purification of DNA from digested samples.
16 BssHII (New England Biolabs) Restriction enzyme to digest viral DNA.
17 Litmus 29 vector (New England Biolabs) Vector for cloning of B19 genome.