ParvoDB ID PV005364 GenBank Accession KF956066
Subfamily Parvovirinae Genus Bocaparvovirus
Species Bocaparvovirus primate2 Sequence Length 495
Definition Human bocavirus 2 strain CF313148072 NS1 (NS1) gene, partial cds. Submission Date 2013-12-11
Strain Name CF313148072 Sampling Date 2013-04
Sampling Country France Location France
Submitted By Brebion,A. Submitting Institution Virology, CHU Clermont-Ferrand, 58 rue Montalembert, Clermont-Ferrand 63000, France
Original Host Homo sapiens Sample Type nasal swab
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Formalin Used for tissue fixation to preserve tissue specimens from all major organs. 24371238 View Related Publication
2 Paraffin Used to embed formalin-fixed tissue specimens.
3 Hematoxylin-eosin Used for staining sections of paraffin-embedded tissue for histological examination.
4 Universal viral transport medium Used to transport collected nasal swab for respiratory virus screening.
5 NucliSENS EasyMAG automated system (bioMérieux, Marcy l’Etoile, France) Used for nucleic acid extraction from nasal swab and various body samples.
6 Respiratory MultiWell System (MWS) r-gene (bioMérieux) Used for real-time RT-PCR screening of respiratory viruses.
7 Nucli-Sens EasyQ Enterovirus kit (bioMérieux) Used for specific enterovirus genome detection by NASBA.
8 QiAamp DNA blood extraction kit (Qiagen, France) Used for nucleic acid extraction from paraffin-embedded myocardial and lung tissue after removal of paraffin with xylene.
9 Xylene Used for removal of paraffin from tissue sections before nucleic acid extraction.
10 Big Dye Terminators v1.1 kit (Applied Biosystems, France) Used for direct sequencing of purified PCR products.
11 r-gene real-time PCR kits (bioMérieux) Used for PCR screening of herpes simplex viruses 1 and 2, cytomegalovirus (CMV), Epstein-Barr virus, and human herpes virus-6 in plasma, urine, and tissue samples.
12 Cobas (Roche Diagnostics) Used for serological tests for CMV and HIV.
13 Serion classic EIA IgG (Virion/Serion) Used for serological tests for Mycoplasma pneumonia (IgG detection).
14 Medac IgM (Medac) Used for serological tests for Mycoplasma pneumonia (IgM detection).
15 Liquid chromatography-mass spectrometry (LC-MS) Used to detect any disorder of amino acid metabolism in plasma, urine, and cerebrospinal fluid specimens.
16 Gas chromatography-mass spectrometry (GC-MS) Used to assay very long chain fatty acids and rule out the possibility of peroxisomal disorders.