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1
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QIAamp DNA Mini Kit
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Viral nucleic acids extraction from recycled water and sewage sludge concentrates.
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35367361
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View Related Publication
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2
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QIAamp RNA Extraction Kit (QIAGEN GmbH, Hilden, Germany)
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Viral nucleic acids extraction from recycled water and sewage sludge concentrates.
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3
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Primers 2028F [5′GAAATGCTTTCTGCTGYTGAAA-3′]
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First round PCR amplification for nested PCR targeting the sequence of the capsid proteins VP1/VP2 junction of HBoV.
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4
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Primers 2029R [5′GTGGATATACCCACAYCAGAA-3′]
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First round PCR amplification for nested PCR targeting the sequence of the capsid proteins VP1/VP2 junction of HBoV.
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5
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Primers 2030F [5′GGTGGGTGCTT CCTGGTTA-3′]
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Nested PCR amplification targeting the sequence of the capsid proteins VP1/VP2 junction of HBoV.
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6
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Primers 2031R [5′TCTTGRATTTCATTTTCAGACAT-3′]
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Nested PCR amplification targeting the sequence of the capsid proteins VP1/VP2 junction of HBoV.
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7
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Taq polymerase (Invitrogen, Carlsbad, CA, USA)
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Enzymatic amplification of DNA in both first round and nested PCR reactions.
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8
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Nuclease-free water
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Component of PCR reaction mixture.
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9
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QIAquick gel extraction kit (QIAGEN, Valencia, CA, USA)
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Purifying the HBoV DNA product from nested PCR.
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