Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV005277 GenBank Accession M24682
Subfamily Parvovirinae Genus Erythroparvovirus
Species Erythroparvovirus primate1 Sequence Length 5255
Definition Human parvovirus B19-Wi promoter region, partial genome. Submission Date 1993-08-03
Strain Name None Sampling Date
Sampling Country None Location None
Submitted By Blundell,M.C., Beard,C. and Astell,C.R. Submitting Institution Virology 157 (2), 534-538 (1987)
Original Host human Sample Type None
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 B19-Wi viral DNA DNA used for sequencing, molecular cloning, and transcription analysis. 3824909 View Related Publication
2 HeLa whole cell extracts Used in in vitro transcription assays to provide necessary transcription factors and RNA polymerase II.
3 HeLa nuclear cell extracts Alternative extracts used in in vitro transcription assays.
4 Restriction enzymes (BarnHI, Pstl, Smal, Xmnl, Spel) Used to digest B19-Wi DNA at specific sites for cloning and transcription analysis.
5 pAT153 vector Cloning vector used for molecular cloning of B19-Wi DNA fragments.
6 pUC13 vector Another cloning vector used for constructing deletion clones of viral DNA.
7 Exonuclease III Used to generate a series of deletions in viral DNA from the left end.
8 Synthetic deoxyribooligonucleotide primer Used in primer extension to determine the 5′-end of the RNA transcript.
9 Reverse transcriptase Used to extend the oligonucleotide primer to generate complementary DNA (cDNA) from the RNA transcript.
10 [α-32P]UTP Radioactive label used in transcription assays to visualize RNA transcripts by autoradiography.
11 α-amanitin Specific inhibitor of RNA polymerase II used to confirm the dependency of transcription on RNA polymerase II.