Panoramic Parvovirus Strain Discovery Data Chain

Download Strain's Sequence
Feedback for This Record
ParvoDB ID PV005276 GenBank Accession AY903437
Subfamily Parvovirinae Genus Erythroparvovirus
Species Erythroparvovirus primate1 Sequence Length 5106
Definition Human parvovirus B19 strain IM-81 NS1 and VP1 genes, complete cds. Submission Date 2014-05-19
Strain Name IM-81 Sampling Date
Sampling Country Germany Location Germany
Submitted By Blumel,J. Submitting Institution Virology, Paul-Ehrlich-Institut, Paul-Ehrlich-Strasse 51-59, Langen 63225, Germany
Original Host human Sample Type Plasma samples
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Highly viremic plasma donations Used as the source of infectious B19V 16254355 View Related Publication
2 KU812Ep6 cells Permissive erythroid cell line used for virus propagation and experiments
3 RPMI Cell culture medium for KU812Ep6 cells
4 Fetal calf serum (Sigma, St. Louis, Mo.) Supplement for cell culture medium
5 Recombinant erythropoietin (NeoRecormon; Roche, Welvyn Garden, United Kingdom) Supplement for cell culture medium
6 QIAquick Gel Extraction kit (QIAGEN, Hilden, Germany) Purification of PCR products by agarose gel electrophoresis
7 AmpliTaq FS BigDye Terminator kit (Applied Biosystems, Weiterstadt, Germany) For sequencing reactions
8 Ethanol Purification of sequencing products
9 ABI sequencing apparatus (Applied Biosystems, Weiterstadt, Germany) For running purified sequencing products on polyacrylamide gels
10 QIAamp Blood Mini kit (QIAGEN, Hilden, Germany) Isolation of DNA
11 TaqMan PCR Core Reagent kit (Applied Biosystems, Weiterstadt, Germany) Master mix for quantitative real-time PCR
12 Uracil-N-glycosylase and AmpliTaq Gold polymerase (Applied Biosystems, Weiterstadt, Germany) Enzymes for PCR reactions
13 World Health Organization international standard for human parvovirus B19 DNA Calibrate DNA quantification standards
14 mRNA Capture kit (Roche, Mannheim, Germany) Extraction of mRNA
15 One-Step RT-PCR kit (QIAGEN, Hilden, Germany) Reverse transcription and PCR
16 Phosphate-buffered saline (PBS) Washing and preparing cells
17 2x lysis buffer Cell lysis for protein extraction
18 SDS-gel electrophoresis in 8% Tris-glycine gels Separating proteins
19 Nitrocellulose membranes Blotting proteins
20 Monoclonal antibody NCL-Parvo (Chemicon International, Temecula, Calif.) Detecting viral capsid proteins
21 Anti-mouse immunoglobulin G (IgG) peroxidase conjugate Secondary antibody detection
22 ECL plus Western Blotting Detection System (Amersham, Freiburg, Germany) Detecting bound antibodies on membranes
23 EDTA Part of cell lysis mixture
24 sodium laurylsarcosine Part of cell lysis mixture
25 Proteinase K Part of cell lysis mixture
26 Agarose Embedding and separating DNA
27 Nylon membranes (Roche, Mannheim, Germany) Transfer DNA during Southern blotting
28 DIG High Prime kit (Roche, Mannheim, Germany) Labeling DNA probes
29 SSC Transfer buffer for Southern blotting
30 Fab fragments of alkaline phosphatase-labeled anti-DIG antibody (Roche, Mannheim, Germany) Detection of labeled DNA
31 CDP-Star (Roche, Mannheim, Germany) Chemiluminescence substrate
32 Commercial human serum albumin product Base solution for pasteurization experiments
33 NaOH Neutralizing acid in pH treatment experiments
34 Phosphate buffer Buffering in pH treatment experiments
35 1 M HCl Adjusting pH
36 Complement-inactivated sera Neutralization experiments
37 Enzyme-linked immunosorbent assay (ELISA) Detection of anti-B19 IgG and IgM