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1
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Human serum albumin (HSA)
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Used for pasteurization to study the inactivation of B19V Genotypes 1 and 3.
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22339291
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2
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Highly viremic plasma donations
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Sources of infectious B19V Genotypes 1 and 3.
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3
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KU812Ep6 cells
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Used for virus titration and culture assay for infectious B19V detection.
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4
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Fetal calf serum (Sigma)
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Supplement for cell culture propagation.
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5
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Recombinant erythropoietin (NeoRecormon, Roche)
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Supplement for propagation of KU812Ep6 cells.
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6
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Thermoblock (Model 5436, Eppendorf)
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Instrument used to heat commercial 5% HSA to 56.5°C.
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7
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Phosphate-buffered saline (PBS)
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Buffer used for adjusting pH in low-pH treatment experiments.
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8
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1 mol/L HCl
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Used to adjust pH for low-pH treatment experiments.
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9
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1 mol/L NaOH
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Used to neutralize samples after low-pH treatment.
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10
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Reverse transcription (RT)-PCR mix (Qiagen)
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Used for RT-PCR to detect B19V capsid mRNA in virus titration assays.
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11
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mRNA capture kit (Roche)
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Used for mRNA extraction in virus titration assays.
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12
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Primers and probes
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Used for detection and amplification of spliced mRNAs encoding for capsid proteins in RT-PCR assays.
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13
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Normal immunoglobulin G (IgG) preparation
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Used in neutralization assays to test the effectiveness of immunoglobulin preparations against B19V Genotypes 1 and 3.
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14
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Cold ethanol
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Used in the preparation of immunoglobulin as part of the fractionation process.
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15
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Commercial 5% human serum albumin (HSA)
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Matrix used for heat inactivation experiments.
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16
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Ethanol, final concentration 2%
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Used in low-pH treatment matrix to mimic manufacturing conditions.
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