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1
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0.45-μm polyether sulfone membrane filter cartridge (Millipore, Billerica, MA)
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Filtration of raw sewage samples for virus concentration.
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19794058
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View Related Publication
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2
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QIAamp MinElute virus spin kit (Qiagen, Valencia, CA)
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Nucleic acid extraction from sewage samples.
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3
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First-strand synthesis SuperScript III reverse transcription kit (Invitrogen, Carlsbad, CA)
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Synthesis of cDNA from extracted viral RNA.
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4
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Random hexamers
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Priming for first-strand cDNA synthesis.
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5
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ExoSap-IT exonuclease (USB, Cleveland, OH)
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Treatment of PCR products before sequencing.
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6
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Taq DNA polymerase (New England Biolabs)
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Amplification in PCR assays.
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7
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ThermoPol reaction buffer with 2 mM MgSO4 (New England Biolabs)
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Reaction buffer for PCR assays.
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8
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Deoxynucleoside triphosphates (dNTPs)
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Nucleotide substrates for PCR amplification.
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9
|
Primers HelF1, HelR1, HelF2, HelR2
|
Targeting conserved 2C (helicase) region in human cardioviruses in nested PCR.
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10
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Primers DKV-N5U-F1, DKV-N5U-R2, DKV-N5U-F2, DKV-N5U-R3
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Targeting 5' untranslated region (5' UTR) in human cosavirus in nested PCR.
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11
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Primers BocaF1, BocaR1, BocaF2, BocaR2
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Targeting VP1 region in human bocavirus in nested PCR.
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12
|
Primers CV-F1, CV-R1, CV-F2, CV-R2
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Targeting replicase gene in circoviruses in nested PCR.
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