Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV005137 GenBank Accession FJ883560
Subfamily Parvovirinae Genus Tetraparvovirus
Species Tetraparvovirus primate1 Sequence Length 79
Definition Human parvovirus 4 isolate 3E2-7 putative capsid protein gene, partial cds. Submission Date 2009-04-02
Strain Name 3E2-7 Sampling Date 2008
Sampling Country France Location southeastern France, France
Submitted By Biagini,P., Touinssi,M., Brisbarre,N. and de Micco,P. Submitting Institution Service de Virologie Moleculaire, EFS Alpes-Mediterranee, 149, Bd. Baille, Marseille 13005, France
Original Host Homo sapiens Sample Type blood samples
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Vacutainer, SST, Becton Dickinson, Meylan, France Collection of blood samples from volunteers. 20031076 View Related Publication
2 Nucleic acids Extraction for testing PARV4 genomic DNA.
3 5 microliters of the eluted material Used for PARV4 DNA detection in real-time PCR assays.
4 Real-time PCR TaqMan methods (StepOne Plus, Applied Biosystems, Courtaboeuf, France) Detection of PARV4 DNA using PCR primers.
5 Primers (ORF2) Compatible with the detection of 3 virus genogroups.
6 PARV4-O probe Used in PCR amplification; sequence: 5′-FAM-TGTTCAACTTTCTCAGGTCCTACCGCCC-TAMRA-3′.
7 PARV4-N probe Used in PCR amplification; sequence: 5′-FAM-TCCTACYGCCCSCTCCTCCTTCTT-TAMRA-3′.
8 Synthetic template Used in dilution to estimate detection limit of as few as 10 copies of PARV4 DNA per reaction.
9 Cloning and M13 sequencing Confirmation of the origin of randomly selected PCR products from PARV4-O and PARV4-N TaqMan assays.