Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV005132 GenBank Accession HM802326
Subfamily Parvovirinae Genus Tetraparvovirus
Species Tetraparvovirus primate1 Sequence Length 57
Definition Human parvovirus 4 isolate HD181 capsid protein gene, partial cds. Submission Date 2010-07-20
Strain Name HD181 Sampling Date
Sampling Country France Location France
Submitted By Biagini,P., Touinssi,M. and de Micco,P. Submitting Institution Service de Virologie Moleculaire, EFS Alpes-Mediterranee, 149, Bd. Baille, Marseille 13005, France
Original Host Homo sapiens Sample Type blood samples
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Vacutainer, SST, Becton Dickinson, Meylan, France Blood sample collection 21328388 View Related Publication
2 MagNA Pure LC instrument (Roche Diagnostics, Meylan, France) Extraction of total nucleic acids from plasma
3 StepOne Plus, Applied Biosystems, Courtaboeuf, France Real-time PCR for detection of PARV4 DNA
4 TaqMan Fast Universal PCR kit, Applied Biosystems Amplification reactions for real-time PCR
5 PARV4Fwd PCR primer for detecting PARV4 DNA
6 PARV4Rev PCR primer for detecting PARV4 DNA
7 PARV4-N Fluorogenic probe for detecting PARV4 DNA
8 STBoNP-1f PCR primer for detecting HBoV DNA
9 STBoNP-1r PCR primer for detecting HBoV DNA
10 STBoNP-1pr Fluorogenic probe for detecting HBoV DNA
11 Qiaquick Gel extraction kit, Qiagen, Courtaboeuf, France Purification of PCR products from agarose
12 pGEM-T Easy Vector System (Promega, Charbonnieres-les-Bains, France) Cloning PCR products into a vector
13 BigDye Terminator v1.1 Sequencing Kit (Applied Biosystems) Sequencing of cloned PCR products