Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV005007 GenBank Accession M10681
Subfamily Parvovirinae Genus Dependoparvovirus
Species Dependoparvovirus primate1 Sequence Length 145
Definition Adeno-associated virus origin of replication (genome 3' terminus). Submission Date 1993-04-27
Strain Name None Sampling Date
Sampling Country None Location None
Submitted By Berns,K.I., Hauswirth,W.W., Fife,K.H. and Lusby,E. Submitting Institution Cold Spring Harb. Symp. Quant. Biol. 43 (Pt 2), 781-787 (1979)
Original Host human Sample Type None
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 CsCl Used for banding of virus after lysis of infected cells with trypsin and deoxycholate. 226321 View Related Publication
2 Trypsin Used for lysis of infected cells.
3 Deoxycholate Used for lysis of infected cells.
4 Alkaline sucrose Used for sedimentation during DNA purification.
5 HpaII Restriction enzyme used for DNA digestion.
6 HaeIII Restriction enzyme used for DNA digestion.
7 BamHI Restriction enzyme used for DNA digestion.
8 TaqI Restriction enzyme used for DNA digestion.
9 Bacterial alkaline phosphatase Used to remove the terminal phosphate from DNA.
10 T4 polynucleotide kinase Used for labeling the 5' ends of DNA molecules.
11 [Y-32P] ATP Used in terminal labeling of DNA with polynucleotide kinase.
12 Polyacrylamide gels Used for electrophoresis to separate DNA fragments.
13 Bisacrylamide Used in high-resolution gel electrophoresis (component of 20% acrylamide gel).
14 Tris Used in buffer for TaqI digestion.
15 MgCl2 Used in buffer for TaqI digestion.
16 β-mercaptoethanol Used in buffer for TaqI digestion.