Panoramic Parvovirus Strain Discovery Data Chain

Download Strain's Sequence
Feedback for This Record
ParvoDB ID PV005005 GenBank Accession AF043303
Subfamily Parvovirinae Genus Dependoparvovirus
Species Dependoparvovirus primate1 Sequence Length 4679
Definition Adeno-associated virus 2, complete genome. Submission Date 1998-01-15
Strain Name None Sampling Date
Sampling Country None Location None
Submitted By Berns,K.I., Bohenzky,R.A., Cassinotti,P., Colvin,D., Donahue,B.A., Dull,T., Horer,M., Kleinschmidt,J.A., Ruffing,M., Snyder,R.O., Tratschin,J.-D. and Weitz,M. Submitting Institution Cell Genesys Inc., 342 Lakeside Dr., Foster City, CA 94404, USA
Original Host human Sample Type None
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 pTAV-2 plasmid Used as the source plasmid for mutagenesis and generation of wild-type virus stocks. 7996133 View Related Publication
2 pTAV-p plasmid Used to generate a virus mutant intended to replace the RGD motif with RGE.
3 pTAV-d plasmid Used to generate a virus mutant intended to delete the RGD motif.
4 M13mpl8 vector Used for cloning the 2.9 kb HindIII/SstI fragment of the capsid protein sequence for site-directed mutagenesis.
5 Amersham mutagenesis kit Used for performing site-directed mutagenesis following the supplier's instructions.
6 Baculovirus-expressed VP3 protein Used for protein sequencing to confirm the corrected amino acid sequence.
7 Endoproteinase lys C Used for digestion of baculovirus-expressed VP3 protein during protein sequencing.
8 1% NP40, 150 mM-NaCl, 20 mM-Tris-HCl (pH 8), 10 mM-DTT, 5 mM-EDTA Lysis buffer for cell lysis followed by sedimentation during protein sequencing.
9 8 M-urea, 20 mM-Tris-HCl (pH 7.5) Used to resuspend the pellet after sedimentation in protein sequencing.
10 SDS-PAGE Used for separating proteins during gel electrophoresis.
11 Immobilon-P membrane (Millipore) Used for transferring proteins after SDS-PAGE.
12 Ponceau S Used for staining the transferred proteins to visualize VP3 position.
13 200 mM-Tris-HCl (pH 8), 0.2% Tween-20, 5 mM-DTT Incubation buffer for membrane pieces containing VP3.
14 5 M-guanidinium-HCl, 200 mM-Tris-HCl (pH 8), 5 mM-DTT Buffer for digesting membrane-bound polypeptides with endoproteinase lys C.
15 Trifluoroacetic acid Added to digest peptides for purification.
16 Protein sequencer (Applied Biosystems, model 477A) Used for sequencing the purified peptides.
17 Antibodies Various antibodies were used for immunoblotting and immunofluorescence analysis.
18 Peroxidase-conjugated secondary antibodies Used for visualizing capsid proteins in immunoblots via enhanced chemiluminescence detection.
19 Hirt extraction reagents Used to prepare extracts for DNA replication analysis.
20 EVL Western blotting Used for detecting sedimentable capsid proteins after high-speed centrifugation.
21 Rhodamine-conjugated secondary antibodies Used for visualizing bound primary antibodies in immunofluorescence staining.
22 Fluorescein isothiocyanate-conjugated secondary antibodies Used for visualizing bound primary antibodies in immunofluorescence double staining.
23 Radiolabelled NdeI/XbaI fragment Used as a probe in dot-blot hybridization.