Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV004475 | GenBank Accession | MG765358 |
| Subfamily | Parvovirinae | Genus | Erythroparvovirus |
| Species | Erythroparvovirus primate1 | Sequence Length | 462 |
| Definition | Human parvovirus B19 isolate NA75 capsid protein VP2 and capsid protein VP1 genes, partial cds. | Submission Date | 2018-01-06 |
| Strain Name | NA75 | Sampling Date | 2013 |
| Sampling Country | Brazil | Location | in the southeastern region of the state of São Paulo, Brazil, Brazil |
| Submitted By | Di Paola,N., Mesquita,F.S., Oliveira,D.B.L., Villabona-Arenas,C.J., Pour,S.Z., de Sousa-Capra,C., Balarini,K., Fonseca,C.R.T.P. and Zanotto,P.M.A. | Submitting Institution | Microbiology, Institute of Biological Sciences, Universeity of Sao Paulo, Av. Prof. Lineu Prestes, 1374, Sao Paulo, SP 05508-000, Brazil |
| Original Host | Homo sapiens | Sample Type | serum samples |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | NUCLISENS easyMag platform (bioMérieux) | Extraction of RNA and total DNA from serum samples | 30304533 | View Related Publication |
| 2 | LightMix Kit Parvovirus B19 EC (TIB MOLBIOL GmbH, Berlin, Germany) | Quantitative real-time PCR assay for B19V | ||
| 3 | High Pure PCR Template Preparation | Preparation of templates for PCR | ||
| 4 | Cobas z 480 Analyzer (Roche Molecular Diagnostics Pleasanton, California) | Analyzer for quantitative real-time PCR assay | ||
| 5 | Positive control and 'no template control' | Controls included in each PCR run to monitor extraction efficacy and PCR inhibition | ||
| 6 | SERION ELISA classic Parvovirus B19 IgG/IgM/IgA (Institut Virion/Serion, Würzburg, Germany) | Quantitative enzyme immunoassays for detecting IgM and IgG antibodies against B19V |