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1
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QIAamp DNA Mini Kit
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Extraction of DNA from EDTA blood
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33952289
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2
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β-actin PCR assay
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Testing DNA samples for β-actin to ensure the quality of DNA extraction
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3
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Gene Amp PCR system 9700
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Amplification of B19V NS1 gene in nested PCR
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4
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E-gel Imager
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Visualization of electrophoresis amplified products under UV illumination
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5
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Sanger sequencing reagents
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Confirming PCR products from nested PCR using cycle sequencing
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6
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Big Dye Terminator v3.1 Cycle Sequencing Kit
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Cycle sequencing of PCR products
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7
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ABI PRISM 7900 sequencer
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Reading sequencing results
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8
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Real Star Parvovirus B19V system
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Performing real-time PCR to detect human B19V from clinical samples and include a heterologous amplification system (internal control)
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9
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Rotor Gene 5plex HRM real-time platform
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Real-time PCR amplification
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10
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NEB Ultra II kit (Cat. No: E7645L)
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Preparation of whole-genome libraries
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11
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Agencourt AMPure XP bead (Cat.No: A63882)
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Cleaning up the adapters ligated DNA fragments
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12
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Qubit High Sensitivity reagent
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Quantification of prepared libraries
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13
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Hi Seq X10
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Generating 2X150 bp sequence reads at 100X sequencing depth
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