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1
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Direct fluorescent-antibody assay kit (Dako Imagen, Cambridgeshire, UK)
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To detect common respiratory viruses (HRSV, adenovirus, influenza A and B, parainfluenza) after admission.
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16965708
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View Related Publication
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2
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QIAamp viral RNA mini kit (Qiagen, Hilden, Germany)
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To extract viral RNA from each sample.
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3
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F-gene primers
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To detect HMPV through reverse transcription–PCR.
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4
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1-a and 1-b primers
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To detect human coronavirus NL-63 through reverse transcription–PCR, as previously described.
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5
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QIAamp DNA Blood Mini Kit (Qiagen GmbH, Hilden, Germany)
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To extract DNA from the nasopharyngeal aspirates.
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6
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Primers 188F (5′-GACCTCTG-TAAGTACTATTAC-3′) and 542R (5′-CTCTGT-GTTGACTGAATACA G-3′)
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For PCR amplification of the NP1 gene.
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7
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Primers HBoV01.2 (5′-TATGGCCAAGGCAATCGTCCAAG-3′) and HBoV02.2 (5′-GCCGCGTGAACATGAGAAACA-GA-3′)
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For PCR amplification of the NS1 gene.
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8
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QIAquick (Qiagen GmbH)
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To purify the amplified DNA fragments.
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9
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BigDye Terminator v3.1 Cycle Sequencing kit (Applied Biosystems, Foster City, CA, USA)
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To sequence the purified amplicons in both directions.
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