Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV003761 GenBank Accession DQ340227
Subfamily Parvovirinae Genus Bocaparvovirus
Species Bocaparvovirus primate1 Sequence Length 245
Definition Human bocavirus strain KR-MN0484 NS1 gene, partial cds. Submission Date 2005-12-21
Strain Name KR-MN0484 Sampling Date from 2004-07 to 2005-10
Sampling Country South Korea Location South Korea
Submitted By Chung,J.Y., Han,T.H., Kim,C.K. and Kim,S.W. Submitting Institution Department of Pediatrics, Inje University Sanggye-Paik Hospital, 761-1 Sanggye7-Dong, Nowon-Gu, Seoul 139 707, South Korea
Original Host None Sample Type nasopharyngeal samples
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Direct fluorescent-antibody assay kit (Dako Imagen, Cambridgeshire, UK) To detect common respiratory viruses (HRSV, adenovirus, influenza A and B, parainfluenza) after admission. 16965708 View Related Publication
2 QIAamp viral RNA mini kit (Qiagen, Hilden, Germany) To extract viral RNA from each sample.
3 F-gene primers To detect HMPV through reverse transcription–PCR.
4 1-a and 1-b primers To detect human coronavirus NL-63 through reverse transcription–PCR, as previously described.
5 QIAamp DNA Blood Mini Kit (Qiagen GmbH, Hilden, Germany) To extract DNA from the nasopharyngeal aspirates.
6 Primers 188F (5′-GACCTCTG-TAAGTACTATTAC-3′) and 542R (5′-CTCTGT-GTTGACTGAATACA G-3′) For PCR amplification of the NP1 gene.
7 Primers HBoV01.2 (5′-TATGGCCAAGGCAATCGTCCAAG-3′) and HBoV02.2 (5′-GCCGCGTGAACATGAGAAACA-GA-3′) For PCR amplification of the NS1 gene.
8 QIAquick (Qiagen GmbH) To purify the amplified DNA fragments.
9 BigDye Terminator v3.1 Cycle Sequencing kit (Applied Biosystems, Foster City, CA, USA) To sequence the purified amplicons in both directions.