Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV003680 GenBank Accession AF085716
Subfamily Parvovirinae Genus Dependoparvovirus
Species Dependoparvovirus mammalian1 Sequence Length 4642
Definition Adeno-associated virus 5 DNA binding trs helicase (Rep22) and capsid protein (VP1) genes, complete cds. Submission Date 1998-08-21
Strain Name None Sampling Date
Sampling Country None Location None
Submitted By Chiorini,J.A., Kim,F., Yang,L. and Kotin,R.M. Submitting Institution MHB, NHLBI, NIH, 10 Center Dr., Building 10, 7D18, Bethesda, MD 20892, USA
Original Host None Sample Type None
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Dulbecco’s modified Eagle’s medium (D10 medium) Maintenance of cos and HeLa cells as monolayer cultures. 9882336 View Related Publication
2 10% fetal calf serum Supplement for D10 medium.
3 Penicillin (100 mg/ml) Antibiotic in the D10 medium to prevent bacterial contamination.
4 Streptomycin (100 U/ml) Antibiotic in the D10 medium to prevent bacterial contamination.
5 Fungizone Antifungal agent included in the D10 medium as recommended by GIBCO.
6 AAV5 stocks Source of Adeno-associated virus type 5; gift from Ursula Bantel-Schaal.
7 Proteinase K Digestion of concentrated virus to isolate viral DNA.
8 Phenol-chloroform Extraction of viral DNA.
9 Ethanol Precipitation of viral DNA.
10 T4 DNA polymerase Creating blunt ends of the viral DNA.
11 XhoI Digestion of viral DNA.
12 Plasmid NEB193 Cloning vector used for inserting viral DNA fragments.
13 ABI 373A automated sequencer and FS dye-terminator chemistry (ABI) Sequencing the viral DNA.
14 Amersham radiolabeled dye-terminator chemistry Sequencing the highly structured ITR region.
15 SalI-SmaI Cloning sites on plasmid NEB193.
16 Plasmid pUC/SV40ori Cloning vector used to subclone large fragments of the AAV5 genome.
17 Plasmid pAV2 Backbone sequence for assembling the AAV5 vector plasmid.
18 Plasmid pAAVRnLacZ Source for the beta-galactosidase expression cassette.
19 X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside) Staining transduced cells for beta-galactosidase activity.
20 Heparin (Sigma H-3149) Competitive inhibitor in heparin competition assay.
21 CsCl Isopycnic gradient centrifugation for purifying viral particles.
22 Adenovirus Helper virus for recombinant AAV particle production.