Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV003661 | GenBank Accession | KM289126 |
| Subfamily | Parvovirinae | Genus | Protoparvovirus |
| Species | Protoparvovirus primate1 | Sequence Length | 456 |
| Definition | Bufavirus-1 isolate CU-B1493 non-structural protein 1 (NS1) gene, partial cds. | Submission Date | 2014-08-13 |
| Strain Name | CU-B1493 | Sampling Date | 2010-06-05 |
| Sampling Country | Thailand | Location | Thailand |
| Submitted By | Chieochansin,T., Vutithanachot,V., Theamboonlers,A. and Poovorawan,Y. | Submitting Institution | Department of Pediatrics, Center of Excellence in Clinical Virology, Faculty of Medicine, Chulalongkorng University, Bangkok 10330, Thailand |
| Original Host | Homo sapiens | Sample Type | fecal sample |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | Buffered saline (PBS) | Dilution of samples in preparation for centrifugation. | 25951971 | View Related Publication |
| 2 | Ribospin vRD II (GeneAll, Seoul, Korea) | Extraction of total viral nucleic acid from supernatants. | ||
| 3 | PerfectTaq MasterMix (5 PRIME, Darmstadt, Germany) | Nested PCR amplification using primers specific for the NS1 gene of BuV. | ||
| 4 | Primers specific for the NS1 gene of BuV | Amplification of BuV DNA during nested PCR. | ||
| 5 | Expin Gel SV (GeneAll, Seoul, Korea) | Purification of PCR products after agarose gel electrophoresis. | ||
| 6 | Agarose gel | Electrophoresis to separate PCR products. |