ParvoDB ID PV003657 GenBank Accession GU048664
Subfamily Parvovirinae Genus Bocaparvovirus
Species Bocaparvovirus primate2 Sequence Length 5173
Definition Human bocavirus 2 strain CU1557UK, complete genome. Submission Date 2009-09-30
Strain Name CU1557UK Sampling Date 2008-07
Sampling Country United Kingdom Location Edinburgh, United Kingdom
Submitted By Chieochansin,T., Simmonds,P. and Poovorawan,Y. Submitting Institution Pediatrics, Faculty of Medicine, Center of Excellence in Clinical Virology, Chulalongkron University and Hospital, Bangkok 10330, Thailand
Original Host Homo sapiens Sample Type fecal sample
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 GoTaq Buffer (Promega, WI) Used as part of the PCR amplification mixture. 20686798 View Related Publication
2 dNTP (Promega, WI) Used as part of the PCR amplification mixture to provide the nucleotides required for DNA synthesis.
3 Forward Primer Used in the semi-nested PCR for amplification of the target DNA sequence.
4 Reverse Primer Used in the semi-nested PCR for amplification of the target DNA sequence.
5 GoTaq DNA Polymerase (Promega, WI) Used as part of the PCR amplification mixture to catalyze the synthesis of DNA.
6 DNA Template Used as the target DNA in the first-round PCR.
7 Nuclease-free Water Used to dilute the PCR mixture to the final volume in the second-round PCR.
8 BigDye 3.0 (ABI, CA) Used for sequencing the PCR amplification products.