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1
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Transport medium with antibiotics (0.5% BSA, Penicillin G (2 × 106 U/l), Streptomycin 200 mg/l)
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To collect nasopharyngeal (NP) suctions from infants or children with respiratory illness.
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17532505
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View Related Publication
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2
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TRI REAGENT LS (Molecular Research Center, Inc., Cincinnati, OH)
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To extract HBoV DNA from the nasopharyngeal suction samples.
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3
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8 mM NaOH
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To solubilize the extracted HBoV DNA.
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4
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188F: 5′-GAGCTCTGTAAGTACTATTAC-3′ (nt position 2351–2371)
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Forward primer for HBoV detection by PCR.
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5
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542R: 5′-CTCTGTGTTGACTGAATACAG-3′ (nt position 2704–2684)
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Reverse primer for HBoV detection by PCR.
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6
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10 μl of 2.5× Eppendorf masterMix (Eppendorf, Hamburg, Germany)
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PCR mix component for HBoV DNA amplification.
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7
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Nuclease-free water
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To adjust the final volume of PCR reaction mixture.
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8
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Various primers as listed in Table 1 such as F5′, NS1R1, R5′, NS1F1, NP1R2, etc.
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Primers for complete coding sequence amplification of HBoV.
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9
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2% agarose gel
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For gel electrophoresis to analyze PCR products.
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10
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Perfectprep Gel Cleanup kit (Eppendorf, GmbH, Germany)
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To purify PCR products from the agarose gel.
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