Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV003635 | GenBank Accession | EF690643 |
| Subfamily | Parvovirinae | Genus | Bocaparvovirus |
| Species | Bocaparvovirus primate1 | Sequence Length | 1182 |
| Definition | Human bocavirus isolate CU25 VP1 gene, complete cds. | Submission Date | 2007-06-20 |
| Strain Name | CU25 | Sampling Date | 2016-07-23 |
| Sampling Country | Thailand | Location | Bangkok, Thailand |
| Submitted By | Chieochansin,T., Chutinimitkul,S., Payungporn,S., Hiranras,T., Samransamruajkit,R., Theamboolers,A. and Poovorawan,Y. | Submitting Institution | Pediatrics, Faclty of Medicine, Chulalongkron University, Center of Excellence in Clinical Virology, Pathumwan, Bangkok, Bangkok 10330, Thailand |
| Original Host | None | Sample Type | Clinical samples |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | TRI REAGENT LS | DNA and RNA extraction from nasopharyngeal suctions. | 18164764 | View Related Publication |
| 2 | 8 mM NaOH | Solubilization of DNA. | ||
| 3 | DepC treated water | Solubilization of RNA. | ||
| 4 | M-MLV reverse transcriptase (Promega) | Reverse transcription (RT). | ||
| 5 | 5X M-MLV reaction buffer (Promega) | Reaction buffer for RT. | ||
| 6 | 10 mM dNTP (Promega) | Nucleotide mix for RT. | ||
| 7 | RNasin Ribonuclease Inhibitor (Promega) | Inhibition of RNase activity during RT. | ||
| 8 | Random Primer (Promega) | Priming during RT. | ||
| 9 | Eppendorf masterMix | PCR reaction mix for HBoV and other respiratory viruses detection. | ||
| 10 | Ethidium bromide | Staining of agarose gels for visualization of PCR products. | ||
| 11 | SYBR Green | Real-time PCR detection. | ||
| 12 | Perfectprep Gel Cleanup kit (Eppendorf) | Purification of PCR products. |