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1
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QIAamp Viral RNA Mini Kit (Qiagen)
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To extract viral RNA and DNA from stool samples.
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18532891
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2
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IDEIA Rotavirus (Dako)
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To detect rotavirus antigen using ELISA.
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3
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Nested PCR with type-specific primers (for G and P genotyping)
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To genotype positive rotavirus samples.
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4
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Multiplex RT-PCR and PCR with three sets of primers [set A, B, and C]
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To identify six enteric viruses: noroviruses GI and GII, sapoviruses, astroviruses, adenoviruses, and group B and C rotaviruses.
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5
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Forward primer HBOV-1 (5′-TATGGCCAAGGCAATCGTCCAAG-3′) and reverse primer HBOV-2 (5′-GCCGCGTGAACATGAGAAACAGA-3′)
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To detect HBoV in the extracted DNA by PCR.
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6
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Forward primer VP1/2-1 (5′-GGACCACAGTCATCAGAC-3′) and reverse primer VP1/2-2 (5′-CCACTACCATCGGGCTG-3′)
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To amplify the partial VP1/VP2 gene of HBoV.
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7
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ExTaq DNA polymerase (Takara Bio)
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To perform PCR amplification.
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8
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QIAquick PCR Purification Kit (Qiagen)
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To purify positive PCR products.
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9
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Forward primer 5′-TGCAGACAACGYTAGTTGTTT-3′ and reverse primer 5′-CTGTCCCGCCCAAGATACA-3′ and probe 5′-CCAGGATTGGGTGGAACTGC-3′
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To quantify HBoV loads using real-time PCR.
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10
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pGEM-T Easy vector (Promega) containing the HBoV NS1 gene
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To serve as a quantification standard for real-time PCR.
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