Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV003572 | GenBank Accession | GU301659 |
| Subfamily | Parvovirinae | Genus | Bocaparvovirus |
| Species | Bocaparvovirus primate2 | Sequence Length | 623 |
| Definition | Human bocavirus 2 isolate LZ53812 VP1 gene, partial cds. | Submission Date | 2009-12-10 |
| Strain Name | LZ53812 | Sampling Date | 2007-08 |
| Sampling Country | China | Location | Gansu, China |
| Submitted By | Cheng,W.-X. and Duan,Z.-J. | Submitting Institution | Viral Diarrhea Department, National Institute for Viral Disease Control and Prevention CDC, China, Ying-Xin Rd.100, Xuan Wu District, Beijing 100052, China |
| Original Host | Homo sapiens | Sample Type | fecal sample |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | PBS (pH7.2) | To make a 10% suspension of stool samples. | 21345238 | View Related Publication |
| 2 | QIAamp Viral RNA Mini kit (Qiagen, Hilden, Germany) | To extract viral RNA and DNA from stool suspensions. | ||
| 3 | Primers HBoV2-sf1, HBoV2-sr1, HBoV2-sf2, HBoV2-sr2 | To amplify a 495-nt region within the ORF of NS1 for detection of HBoV, HBoV2, HBoV3, and HBoV4. | ||
| 4 | Primers NP1-F1, NP1-R1, NP1-F2, NP1-R2 | To amplify part of the NP1 gene in positive samples. | ||
| 5 | Primers VP1-F1, VP1-R1, VP1-F2, VP1-R2 | To amplify part of the VP1 gene in positive samples. | ||
| 6 | ExTaq DNA polymerase (Takara Bio) | Used in the PCR reaction mix for the amplification of NP1 and VP1 genes. | ||
| 7 | Genome Walking Kit (TaKaRa code: D316) | Used to amplify complete HBoV2 sequences. |