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1
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Human alveolar epithelial A549 cells
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Cell line used for transfection experiments.
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20457462
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2
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Human embryonic kidney 293 cells
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Cell line used for transfection and replication experiments.
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3
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Lipofetamine and Plus reagent (Invitrogen)
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Transfection reagent for introducing plasmid DNA into cells.
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4
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LipoD293 transfection reagent (SignaGen Laboratories)
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Transfection reagent for introducing plasmid DNA into cells.
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5
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Trizol reagent (Invitrogen)
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Reagent for isolation of total RNA from cells.
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6
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FastTrack MAG Micro mRNA isolation kit (Invitrogen)
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Kit for mRNA purification from total RNA.
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7
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pGEM4Z vector (Promega)
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Vector used for probe generation in transcription mapping.
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8
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32P-labeled RNA markers
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Markers used for determining RNA size in RNase protection assay.
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9
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RNA maker ladder (Ambion)
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Ladder used for determining RNA size in Northern blot analysis.
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10
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pcDNA3 vector (Stratagene)
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Plasmid vector used for cloning and expression of tagged constructs.
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11
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GST expression system components (GE Health)
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System used for generating antigens for antibody production.
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12
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Anti-HA antibody (Sigma)
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Antibody used for detecting HA-tagged constructs in Western blot.
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13
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Antiserum against HBoV1 NS1, NP1, VP1/2
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Produced for detecting specific HBoV1 proteins in Western blot and immunofluorescence assays.
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14
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FLICA peptides (Immunochemistry Tech, MN)
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Reagent used for detecting active caspases in apoptosis assays.
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15
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DAPI
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Staining of cell nuclei for cell cycle analysis by flow cytometry.
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16
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Cy5-labeled secondary antibody
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Secondary antibody used for detecting primary antibodies in flow cytometry and immunofluorescence assays.
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