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1
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Lipofectamine
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Used for transfection of COS-7 cells.
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19758675
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2
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Plus reagent
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Used for transfection of COS-7 cells.
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3
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DMEM (Dulbecco's modified Eagle's medium)
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Used to maintain COS-7 and UT7/Epo-S1 cells.
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4
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Fetal calf serum (FCS)
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Supplement used in DMEM for culturing COS-7 and UT7/Epo-S1 cells.
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5
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Epo [Procrit (Epoetin Alfa), Centocor Ortho Biotech Inc.]
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Used as a supplement for culturing UT7/Epo-S1 cells.
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6
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TRIZOL reagent (Invitrogen)
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Used for total RNA isolation from transfected cells.
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7
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MAXIscript kit (Ambion)
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Used for in vitro transcription to generate RNase protection assay (RPA) probes.
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8
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32P-labeled RNA markers
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Used as size markers in RNase protection assay.
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9
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BamHI-HindIII digested pGEM3Z (Promega)
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Vector used for cloning regions for RNase protection probes.
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10
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HindIII/BamHI digested pcDNA3 (Invitrogen)
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Vector backbone used for constructing GFP-fused NS1 constructs.
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11
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DNA maker ladder [Ambion]
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Used as a size marker in Northern blot analysis.
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12
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Anti-CD36 antibodies
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Used for generating primary human CD36+ erythroid progenitor cells (CD36+ EPCs).
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13
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Propidium Iodide (PI, Sigma)
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Used for staining cells to detect apoptosis.
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14
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Cy5-conjugated AnnexinV (BD Biosciences)
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Used for live staining of cells to detect apoptosis.
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15
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FACS DIVA software (BD Biosciences)
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Used for analyzing flow cytometry data.
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