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1
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Dulbecco’s modified Eagle's medium
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Cultured A459 cells for adenovirus isolation.
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16282478
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2
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Fetal calf serum
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Supplemented cell culture medium for adenovirus isolation.
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3
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Digestion buffer (10 mM Tris, pH 8; 100 mM NaCl; 0.5% sodium dodecyl sulfate; 25 mM EDTA) supplemented with 2 mg/ml proteinase K
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For DNA isolation from human tissue samples.
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4
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Phenol-chloroform/isoamyl alcohol (25:24:1)
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For DNA extraction.
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5
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DNase-free RNase A (QIAGEN Inc.)
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To remove RNA from DNA samples.
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6
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[α-32P]dCTP
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Labeled hybridization probe for in-gel Southern blot hybridization.
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7
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SureStart Taq polymerase (Stratagene)
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For PCR amplification.
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8
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TOPO TA cloning kit (Invitrogen, Inc.)
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Cloning PCR products into vectors for sequencing.
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9
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BigDye terminator chemistry
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For sequencing DNA clones.
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10
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Horseradish peroxidase-linked sheep anti-human immunoglobulin G (Sigma Chemical Co.)
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For detecting human antibodies in ELISA.
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11
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5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal)
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Substrate for staining rAAV/β-gal infected cells.
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12
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Taqman PCR master mix
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For quantitative PCR analysis.
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13
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POROS HE-20 heparin column
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To assess the heparin-binding capacity of AAV capsids.
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14
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Sheep anti-human immunoglobulin G
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Secondary antibody for ELISA.
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15
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o-phenylenediamine dihydrochloride (Sigma)
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Substrate for colorimetric detection in ELISA.
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