Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV002391 | GenBank Accession | KP115317 |
| Subfamily | Parvovirinae | Genus | Erythroparvovirus |
| Species | Erythroparvovirus primate1 | Sequence Length | 427 |
| Definition | Human parvovirus B19 isolate RJ900/2000 VP2 gene, partial cds. | Submission Date | 2014-11-06 |
| Strain Name | RJ900/2000 | Sampling Date | from 1996 to 2006 |
| Sampling Country | Brazil | Location | EI in the municipality of Niterói, Rio de Janeiro state, Brazil |
| Submitted By | Cubel Garcia,R.C.N., Pereira,R.F.A., Azevedo,K.M.L., Castro,T.X., Mello,F.C.A., Setubal,S., Siqueira,M.M.A.T. and Oliveira,S.A. | Submitting Institution | Departamento de Microbiologia e Parasitologia, Universidade Federal Fluminense, Rua Hernani de Melo 101, Niteroi, Rio de Janeiro, Brazil |
| Original Host | Homo sapiens | Sample Type | serum samples |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | QIAamp DNA Blood Mini Kit (QIAGEN, Hilden, Germany) | Viral DNA extraction from serum samples | 27914222 | View Related Publication |
| 2 | Enzyme immunoassay (Biotrin International, Dublin, Ireland) | Testing presence of anti-B19V IgM | ||
| 3 | Primer pairs P12/P16 and P13/P16 | Partial amplification of the VP1/VP2 capsid gene for genotyping B19V strains | ||
| 4 | Illustrative GFXTM PCR DNA and Gel Band Purification kit (GE, Healthcare, Buckinghamshire, UK) | Purification of PCR amplicons | ||
| 5 | BigDye terminator v. 1.1 cycle sequencing kit (Applied Biosystems, CA, USA) | Direct sequencing of PCR amplicons | ||
| 6 | ABI Prism 3730 DNA analyzer (Applied Biosystems, CA, USA) | Sequencing analysis |