Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV002305 | GenBank Accession | MN765166 |
| Subfamily | Parvovirinae | Genus | Dependoparvovirus |
| Species | Dependoparvovirus primate1 | Sequence Length | 1972 |
| Definition | MAG: Dependoparvovirus primate1 isolate SAfia-074D VP1 gene, partial cds. | Submission Date | 2019-12-03 |
| Strain Name | SAfia-074D | Sampling Date | from 2014-12 to 2016-02 |
| Sampling Country | United Republic of Tanzania | Location | outpatient clinics, United Republic of Tanzania |
| Submitted By | Cordey,S., Laubscher,F., Hartley,M.-A., Junier,T., Keitel,K., Docquier,M., Guex,N., Iseli,C., Vieille,G., Le Mercier,P., Gleizes,A., Samaka,J., Mlaganile,T., Kagoro,F., Masimba,J., Said,Z., Temba,H., Tapparel,C., Zanella,M.-C., Xenarios,I., Fellay,J., D'Acremont,V. and Kaiser,L. | Submitting Institution | Laboratory of Virology, University Hospitals of Geneva, Rue Gabrielle-Perret-Gentil 4, Geneva, Geneva 1205, Switzerland |
| Original Host | Homo sapiens | Sample Type | sera samples |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | Turbo DNAse | DNAse treatment of serum samples to degrade DNA. | 33929935 | View Related Publication |
| 2 | 10X DNAse Buffer | Buffer used in the DNAse treatment of serum samples. | ||
| 3 | TRIzol | Isolation of RNA genomes. | ||
| 4 | NucliSens easyMAG magnetic bead system | DNA virus genome extraction. | ||
| 5 | DNA polymerase I, Large Fragment (Klenow) | Double-stranded DNA synthesis. | ||
| 6 | Nuclease-free water | Resuspension of RNA and DNA pellets. | ||
| 7 | Qubit 3.0 Fluorometer | Quantify RNA and DNA concentrations. | ||
| 8 | Ribo-Zero Gold depletion kit | Ribosomal RNA removal from RNA samples. | ||
| 9 | TruSeq total RNA preparation protocol | RNA library preparation for sequencing. | ||
| 10 | HiSeq 2500 platform | Multiplexed sequencing of RNA libraries. | ||
| 11 | Nextera XT protocol | DNA library preparation for sequencing. | ||
| 12 | HiSeq 4000 platform | Multiplexed sequencing of DNA libraries. | ||
| 13 | TapeStation (Agilent) | Control of the size distribution of fragments in RNA and DNA libraries. | ||
| 14 | Canine distemper virus (CDV)-positive control | Positive control for RNA virus detection. | ||
| 15 | Baculovirus (GenScript) | Spiked positive control for DNA virus detection. |