Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV002294 GenBank Accession HQ245658
Subfamily Parvovirinae Genus Tetraparvovirus
Species Tetraparvovirus primate1 Sequence Length 132
Definition Human parvovirus 4 isolate 103 non-structural protein (ORF1) gene, partial cds. Submission Date 2010-09-08
Strain Name 103 Sampling Date from 2006 to 2010
Sampling Country Italy Location Italy
Submitted By Corcioli,F., Zakrzewska,K. and Azzi,A. Submitting Institution Public Health, University of Firenze, Viale Morgagni 48, Firenze, FI 50134, Italy
Original Host Homo sapiens Sample Type Clinical samples
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 High Pure PCR Template Preparation Kit (Roche, Basel, Switzerland) DNA extraction 20950445 View Related Publication
2 Primers PVORF2F and PVORF2R Real-time PCR targeting the ORF2 region of PARV4 for virus detection and viral load assessment
3 Quantitec SYBR Green PCR Master Mix (Qiagen, Hilden, Germany) PCR mixture for real-time PCR analysis
4 Rotor-Gene 3000 real-time apparatus (Corbett Research, Sidney, Australia) Conducting real-time PCR reactions
5 p-GEM-T vector system (Promega, Milano, Italy) Constructing the calibration curve for real-time PCR
6 Primers targeting a conserved region of ORF1 Performing a nested PCR for confirmation of PARV4 DNA detection
7 Primers for β-globin gene Real-time PCR for calculating the number of cells in the tissue extracts
8 ABI PRISM Dye Terminator Cycle Sequencing Ready Reaction Kit Sequencing of PCR amplicons
9 Primers PVORF2F and PVORF2R (for ORF2) and inner primers (for ORF1) Sequencing amplicons for genotyping of PARV4 and verification