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1
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High Pure PCR Template Preparation Kit (Roche, Basel, Switzerland)
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DNA extraction from tissue and serum samples.
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18814251
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2
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TriPure Isolation Reagent (Roche, Basel, Switzerland)
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RNA extraction from tissue samples.
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3
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Taq DNA polymerase
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Ensure the integrity of DNA and check for presence of inhibitors.
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4
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Beta-globin primers
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PCR to check DNA integrity and absence of PCR inhibitors.
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5
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RT-PCR for cellular β-actin mRNA
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Check the efficiency of RNA extraction.
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6
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Primers 1905f and 1987r
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Consensus PCR for B19V detection targeting NS1 gene.
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7
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Primers e2717f and e2901r
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Consensus PCR for B19V detection targeting VP1 unique region.
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8
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Mfe I and Apa I
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Restriction Fragment Length Polymorphism (RFLP) analysis for genotyping.
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9
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Genotype 1 specific nested PCR primers
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Genotype-specific PCR for B19V detection.
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10
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Genotype 2 specific PCR primers
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Genotype-specific PCR for B19V detection.
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11
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BigDye Terminator Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA)
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Sequencing of viral DNA for genotype determination.
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12
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Primers for real-time PCR with Syber Green
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Real-time PCR to quantify viral load using Syber Green dye.
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13
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Quantitect SYBR Green PCR Master Mix (Qiagen, Hilden, Germany)
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Real-time PCR for quantifying B19V DNA.
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14
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pGEM-T vector (Promega, Milano, Italy)
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Cloning of PCR amplification products for creating calibration curves.
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15
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B19-DNA ISS 0300 (Istituto Superiore di Sanita, Roma, Italy)
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Evaluating the reliability of PCR reactions.
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16
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RT-PCR for β-globin gene
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Calculate the number of cells extracted from tissues.
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17
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RT-PCR for viral mRNA detection
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Detect viral mRNAs for structural and non-structural proteins.
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18
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TaqGold DNA polymerase (Applied Biosystems, Foster City, CA, USA)
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High-efficiency PCR amplification.
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