1
Monoclonal antibodies
Used for direct immunofluorescence assays to test samples for common viral respiratory pathogens such as RSV, influenza A and B viruses, parainfluenza type 1, 2, and 3 viruses, and human adenovirus.
16965707
2
MRC-5 cell monolayers
Used for virus isolation.
3
Primers targeting the predicted NP1 gene
Used to test nucleic acid extracts for HBoV DNA by PCR.
4
pGEM-T Easy Vector (Promega)
Used for cloning the 354-bp NP1 PCR fragment to obtain the HBoV-NP1 plasmid, which served as control in a subsequent real-time PCR assay.
5
LightCycler 2.0 (Roche Diagnostics)
Used for performing the real-time PCR assay.
6
Primers BocaRT1 and BocaRT2
Used in the real-time PCR assay to quantitate HBoV DNA in positive samples.
7
FAM/TAMRA dually labeled probe
Used in the real-time PCR assay for detecting PCR amplification.
8
FastStart DNA Hybridization Mix (Roche Diagnostics)
Used for PCR amplification in the real-time PCR assay.
9
pGEM-T HBoV NP1 plasmid
Used for generating standard curves in the PCR quantitation assay.
10
LightCycler control DNA kit (Roche Diagnostics)
Used for measuring DNA levels in HBoV-positive samples.
11
Primers BocaSEQ1, BocaSEQ4, BocaSEQ2, and BocaSEQ3
Used for amplification and sequencing of a 1-kb DNA fragment encompassing the VP2 gene.
