Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV000788 | GenBank Accession | HM485563 |
| Subfamily | Parvovirinae | Genus | Bocaparvovirus |
| Species | Bocaparvovirus primate1 | Sequence Length | 324 |
| Definition | Human bocavirus isolate CIT-H258 viral protein 1 (VP1) gene, partial cds. | Submission Date | 2010-06-10 |
| Strain Name | CIT-H258 | Sampling Date | from 2006 to 2008 |
| Sampling Country | Ireland | Location | the Munster region of Southern Ireland, Ireland |
| Submitted By | Cashman,O. | Submitting Institution | Biological Sciences, Cork Institute of Technology, Rossa Avenue, Bishopstown, Cork, Munster, Ireland |
| Original Host | Homo sapiens | Sample Type | Stool samples and non-bacterial gastroenteritis samples |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | QIAamp DNA Blood Mini Kit (QIAGEN GmbH, Hilden, Germany) | DNA purification from faecal samples | 22614812 | View Related Publication |
| 2 | Primers sf1/sr1 and sf2/sr2 | Targeting the NS region of HBoV and HBoV2 for PCR analysis | ||
| 3 | Pan-bocavirus primers AF.VP1/2 F1 AF.VP1/2 R1, and AF.VP1/2F2 AF.VP1/2 R2 | Amplification of the VP1/2 gene of HBoV1, 2, 3, and 4 for PCR analysis | ||
| 4 | Positive control, pBoca2 | Control for PCR assays; contains all three bocavirus genes | ||
| 5 | High Pure PCR Product Purification Kit (Roche Diagnostics GmbH, Mannheim, Germany) | Purification of amplicons from positive samples for sequencing |