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1
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Secreción respiratoria (aspirado nasofaríngeo)
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Used as a sample obtained from a pediatric patient for isolation of HBoV1.
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26913800
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View Related Publication
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2
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Buffer de guanidina
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Used in the extraction of nucleic acids.
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3
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Precipitación con sílica
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Used for nucleic acids precipitation.
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4
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Buffer TE (10:1)
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Used to resuspend the nucleic acid extract.
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5
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PCR reactivos de la firma Invitrogen
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Initial reagents for PCR including 2.5 mM MgCl2, 0.8 mM equimolar dNTPs mix, 0.02 U/μL Taq DNA polymerase, and 0.4 μM of each primer.
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6
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Enzima Platinum Taq DNA Polymerase High Fidelity (Invitrogen)
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Used in the final PCR assays for genome sequencing with high fidelity.
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7
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Poliacrilamida al 8.5%
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Used in gels for visualizing PCR products stained with silver nitrate.
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8
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Gel de agarosa al 1.5%
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Used for visualizing PCR products stained with ethidium bromide.
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9
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Qiagen PCR cleanup kit
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Used for purifying amplification products before sequencing.
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10
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BigDye Terminator v. 3.1 (Applied Biosystems)
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Used for bidirectional sequencing of amplified fragments.
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11
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Secuencias representativas de GenBank
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Used for the alignment and design of primers.
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