Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV000590 | GenBank Accession | GU265899 |
| Subfamily | Parvovirinae | Genus | Bocaparvovirus |
| Species | Bocaparvovirus primate1 | Sequence Length | 774 |
| Definition | Human bocavirus strain I-MI/04-04 VP1 protein (VP1) and VP2 protein (VP2) genes, partial cds. | Submission Date | 2009-12-03 |
| Strain Name | I-MI/04-04 | Sampling Date | from 2002 to 2007 |
| Sampling Country | Italy | Location | Italy |
| Submitted By | Canuti,M., Zappa,A. and Tanzi,E. | Submitting Institution | Public Health-Microbiology-Virology, University of Milan, Via Pascal 36, Milan 20133, Italy |
| Original Host | Homo sapiens | Sample Type | pharyngeal swabs |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | DNA Mini Kit (QIAGEN GmbH, Germany) | Extraction of HBoV-DNA from pharyngeal swabs. | 19932194 | View Related Publication |
| 2 | Taq polymerase (GoTaq, Promega, Madison, WI) | Used in the first amplification step of PCR. | ||
| 3 | Primers (VP s1 and VP as1) | Used in the first amplification step of PCR. | ||
| 4 | dNTPs (Promega) | Used in the first amplification step of PCR. | ||
| 5 | PCR buffer | Used in the first amplification step of PCR. | ||
| 6 | Primers (VP s2 and VP as1) | Used in the second amplification step of semi-nested PCR. | ||
| 7 | GeneAmp PCR System 9700 thermal cycler (Applied Biosystem, Foster City, CA) | Thermal cycling for the PCR amplification steps. | ||
| 8 | NucleoSpin Extract II (Macherey-Nagel GmbH, Germany) | Purification of the amplified fragments. | ||
| 9 | ABI PRISM 3100 genetic analyser (Applied Biosystems, Foster City, CA) | Automated DNA sequencing. |