Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV000103 GenBank Accession AY582146
Subfamily Parvovirinae Genus Erythroparvovirus
Species Erythroparvovirus primate1 Sequence Length 863
Definition B19 virus strain Gh1280R NS1 and VP1u genes, partial cds. Submission Date 2004-03-25
Strain Name Gh1280R Sampling Date from 1999 to 2003
Sampling Country Ghana Location the United Kingdom and sub-Saharan Africa (Ghana, Malawi, and South Africa), Ghana
Submitted By Candotti,D., Etiz,N., Parsyan,A. and Allain,J.-P. Submitting Institution Cambridge Blood Centre, National Blood Service, Long Road, Cambridge, Cambridgeshire CB2 2PT, United Kingdom
Original Host Human Sample Type Plasma samples
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 High Pure viral nucleic acid kit Isolation of viral DNA from plasma 15507603 View Related Publication
2 AmpliTaq (Applied Biosystems) First PCR round enzyme for amplifying viral DNA
3 Mx4000 multiplex quantitative PCR (Q-PCR) system (Stratagene) Quantification of viral DNA
4 Brilliant quantitative PCR core reagent kit (Stratagene) Core reagents for performing Q-PCR
5 First International Standard for Parvovirus B19 DNA NAT assays 99/800 (NIBSC) Reference curve for quantification in Q-PCR
6 TaqMan-based methodology Primers and probe design for Q-PCR detection of viral DNA
7 Plasmid pD91.1-C10 Reference sample expressing genotype 3 V9-related strain D91.1
8 QIAquick gel extraction kit (Qiagen) Purification of PCR amplicons
9 ABI PRISM BigDye terminator cycle sequencing ready reaction kit (version 3.0) Sequencing of purified amplicons
10 ABI PRISM 3100 genetic analyzer (Applied Biosystems) Sequence analysis of viral DNA
11 Biotrin parvovirus B19 IgM and IgG EIAs Detection of antibodies to erythroviruses
12 Protein G-Sepharose (Amersham Pharmacia Biotech) Affinity chromatography separation of free and IgG-associated viruses
13 Purified recombinant VP1 protein Detection of antibodies to VP2 antigen of erythrovirus variant V9