Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV000097 | GenBank Accession | KM366090 |
| Subfamily | Parvovirinae | Genus | Bocaparvovirus |
| Species | Bocaparvovirus primate2 | Sequence Length | 507 |
| Definition | Human bocavirus isolate BR/UFBA/LabViro/292_E6-2012 viral protein 1 (VP1) gene, partial cds. | Submission Date | 2014-08-18 |
| Strain Name | BR/UFBA/LabViro/292_E6-2012 | Sampling Date | from 2012-01 to 2012-07 |
| Sampling Country | Brazil | Location | the emergency pediatric hospital in Salvador, city of Bahia, Brazil, Brazil |
| Submitted By | Campos,G.S., Sardi,S.I., Brandao,C.J.F., Sampaio,M.L.S., Menezes,A.D.L., Chinalia,F.A., Costa,L.F.M., Tigre,D.M. and Paula,F.L. | Submitting Institution | Bio-interaction, Universidade Federal da Bahia, Av Reitor Miguel Calmon S/N, Salvador, BA 40110902, Brazil |
| Original Host | Homo sapiens | Sample Type | fecal specimens |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | 105 stool specimens | Sample collection from children with acute gastroenteritis. | 26059266 | View Related Publication |
| 2 | RIDASCREEN 3rd generation Norovirus (R-Biopharm, Germany) | Detection of Norovirus in stool specimens. | ||
| 3 | RIDASCREEN Adenovirus (R-Biopharm, Germany) | Detection of Adenovirus in stool specimens. | ||
| 4 | RIDASCREEN Rotavirus (R-Biopharm, Germany) | Detection of Rotavirus in stool specimens. | ||
| 5 | QIAmp Viral DNA Mini kit (Qiagen, Brazil) | Total DNA extraction from stool samples. | ||
| 6 | Phosphate-buffered saline solution | Dissolve stools before DNA extraction. | ||
| 7 | AK-VP-F1 and AK-VP-R1 primers | First round of nested-PCR targeting HBoV VP1/2 gene. | ||
| 8 | AK-VP-F2 and AK-VP-R2 primers | Second round of nested-PCR targeting HBoV VP1/2 gene. | ||
| 9 | Ethidium bromide | Staining of gel electrophoresis to visualize PCR products. | ||
| 10 | QIAquick PCR purification kit (Qiagen, Brazil) | Purification of PCR products before sequencing. |